The receptor then returns to the cell membrane surface.Ĭhromosomal Location of Human Ortholog: 17p13. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The protein encoded by this gene is the more. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The receptor recognizes terminal galactose and N-acetylgalactosamine units. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.ĪSGR1: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The protein encoded by this gene is the more abundant major subunit. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ASGR1/ASGPR1 Monoclonal Antibody (Catalog MAB43941, filled histogram). The monoclonal antibody 8D7 is cross reactive with human ASGPR. Immunohistochemistry analysis of paraffin-embedded mouse liver using ASGR1 antibody (A16766) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol. Fixed with 4 PFA blocked with 0.3 BSA (30 min). 1X106 HepG2 cells were stained with 0.2ug ASGR1 antibody (11739-1-AP, red) and control antibody (blue). The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. Species Reactivity: Human Immunogen: Mouse myeloma cell line NS0-derived recombinant human ASGR1/ASGPR1. Mouse liver tissue were subjected to SDS PAGE followed by western. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. However, it is unknown exactly how ASGR1. The present invention generally relates to antibodies specific for asialoglycoprotein receptor (ASGPR) and their use for selectively delivering effector. Detection of ASGR1/ASGPR1 in HepG2 Human Cell Line by Flow Cytometry.
This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. Mice lacking Asgr1 were phenotypically normal and the cholesterol-lowering effects of Asgr1 deficiency were recapitulated in mouse and pig 11,12,13. Only those wells that contain Mouse ASGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. This gene encodes a subunit of the asialoglycoprotein receptor.
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